FastQCFastQC Report
Sun 14 Apr 2019
SRR6576560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349887
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6770.19349104139336415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4600.13147101778574224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.12804133906089682No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4230.12089617505080211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9450.046.8099061
TACATGG20750.039.8636672
GTACATG20850.039.272171
ACATGGG21200.038.568613
AACGCAG12200.037.3622066
CAACGCA12700.035.521245
ATCAACG12700.035.521243
TCAACGC13000.034.701524
ATGGGGG3400.033.1705675
CTAGTAC1309.165888E-932.5326733
CATGGGG8750.032.222844
CCTAGTA1351.3220415E-831.332242
ACGCAGA14550.031.327767
TATCAAC14150.030.889382
CGCAGAG15050.030.616718
CATGGGA11400.029.678934
TAGTACT1602.1263986E-929.3697744
CTGTGCG1452.638808E-829.1839089
GTCATAA1205.7714715E-627.4510231
ATGGGAT4800.027.411795
Produced by FastQC (version 0.11.2)