FastQCFastQC Report
Sun 14 Apr 2019
SRR6576561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466792
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7000.14995972510240108No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.1231812027626866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.057.1780971
ATCAACG15450.040.1541983
TCAACGC16200.038.2952044
GTACATG27100.037.9804651
CAACGCA16150.037.831745
TACATGG27450.037.49622
GGTATCA4450.036.965241
ACATGGG27400.036.706983
AACGCAG16850.036.539026
TATCAAC18850.033.1608622
ACGCAGA20500.030.2625527
CGCAGAG20850.029.7545498
CATGGGT6950.029.7545474
CATGGGG12800.029.7413434
CATGGGA10600.029.2633154
TAGGACA1904.456524E-1027.2097514
GCAGAGT23000.026.9731439
ATGGGTA4300.026.2318125
AGAGTAC20700.026.2238910-11
ATGGGGG5250.025.9611645
Produced by FastQC (version 0.11.2)