FastQCFastQC Report
Sun 14 Apr 2019
SRR6576561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466792
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8230.17630979108468012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.16452724125520576No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5730.12275274640525116No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5090.10904214296731735No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4800.10282952578450359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.057.1608851
ATCAACG17100.040.9515343
CAACGCA17750.039.1871225
GTACATG28300.038.6360661
TACATGG28500.038.587892
TCAACGC18200.038.2182124
ACATGGG28400.037.5653573
AACGCAG19100.036.1712886
TCCGGAC651.4125448E-436.1523633
TATCAAC19950.035.101322
CATGGGA13200.032.4001854
CGCAGAG21850.031.4037488
ACGCAGA21950.031.2606757
CATGGGT6250.030.8307364
GGTATCA5500.029.9913961
ATGGGGA5650.028.2820245
ATGGGAG6550.027.9835835
CCTTATA1205.8353016E-627.415542
CATGGGG12900.027.3244614
GCAGAGT25400.027.199679
Produced by FastQC (version 0.11.2)