FastQCFastQC Report
Sun 14 Apr 2019
SRR6576563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497430
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8900.1789196469855055No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5030.10111975554349356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.1001145889873952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.038.3886151
GTACATG25800.034.7939381
TACATGG25850.034.726642
ACATGGG26800.032.969553
AACGCAG14850.032.5987326
TCAACGC15550.031.1312664
CAACGCA15650.030.6320325
ATCAACG15850.030.5420323
ACGCAGA17000.028.4759527
TATCAAC17900.027.8318962
CGCAGAG17500.027.3937878
CATGGGA13000.027.1148934
ATGGGTA3300.027.0601145
GTCCTAG1755.5388227E-926.8566531
CATGGGG11600.026.3357264
CATGGGT6100.025.4257664
TCAATAC1201.8362325E-423.4995733
GCAGAGT21000.023.0519629
GTCTTAT1652.659217E-622.7874641
CTATACT1503.2869983E-521.9329344
Produced by FastQC (version 0.11.2)