FastQCFastQC Report
Sun 14 Apr 2019
SRR6576563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497430
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7900.1588163158635386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.10031562229861488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.044.385981
TACATGG26000.037.4183122
GTACATG26350.036.112971
ACATGGG26300.035.7405663
ATCAACG15100.032.6813163
TCAACGC15150.032.2632334
CATGGGG10950.032.190994
CAACGCA15200.031.8479025
AACGCAG15400.031.4342926
TATCAAC17600.029.1072372
CCCTATA1005.3184383E-528.1993082
CATGGGT7050.027.999314
ACGCAGA18100.027.004867
CGCAGAG18500.026.9290668
TAGTACT1606.901064E-826.4368484
CATGGGA13100.025.8314274
ATGGGGA7850.025.7445895
ATGGGTA4150.024.915055
TACCCGT601.8381102E-423.49942274-75
TAGCCCT2803.6379788E-1223.4994224
Produced by FastQC (version 0.11.2)