FastQCFastQC Report
Sun 14 Apr 2019
SRR6576564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446567
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8200.18362306216088514No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4880.10927811504208775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10550.042.775111
GTACATG23250.035.7867851
TACATGG23050.035.485482
GTATAGC1107.6668584E-834.1876831
ATCAACG12800.034.1393
AACGCAG13000.033.9752246
ACATGGG23350.033.8065033
TCAACGC13000.033.6137854
CCGAACT851.7503327E-533.1821631
CAACGCA13550.032.249395
ACGCAGA15750.028.3413727
CATGGGG10400.028.011494
CGCAGAG15950.027.9859948
TATCAAC16100.027.1537272
CATGGGA11400.025.9665074
ATGGGTA3100.025.767075
GTAATAG950.001302033524.7410871
GCAGAGT18550.024.3167299
CATGGGT5600.023.4935054
TATAGTA1252.4174039E-422.5638712
Produced by FastQC (version 0.11.2)