FastQCFastQC Report
Sun 14 Apr 2019
SRR6576567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272678
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4400.16136248615583215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.10818621230902384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7300.045.076581
CTCTATA608.7970664E-539.17372
CTATACC608.8065804E-539.1665154
AACGCAG8750.036.5255746
TACATGG15750.036.4128882
ACATGGG16100.035.614773
GTACATG16400.035.5429651
TCAACGC8950.035.184224
ATCAACG9100.034.604263
CAACGCA9350.033.6790125
GCAAACG851.7477496E-533.1824261
CATGGGG7150.032.2096634
ACTATAC600.00415554731.3332123
ACGCAGA10100.030.7127517
CGCAGAG10350.029.9708988
TATCAAC11350.029.4061582
CAAACGC856.795528E-427.6520212
CTATACA856.802821E-427.6469544
AGTAGGG856.802821E-427.6469545
ATGGGGA3950.027.3669825
Produced by FastQC (version 0.11.2)