FastQCFastQC Report
Sun 14 Apr 2019
SRR6576567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272678
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3820.14009197661710882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.11918820000146693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6400.042.6556661
TAATAGG802.4496694E-741.124364
TACATGG15250.038.523992
GTACATG15800.037.8333931
ACATGGG15450.037.7210923
ATCAACG8450.032.2598533
CATGGGG6400.031.5776354
TCAACGC8500.031.5171574
AACGCAG8500.030.9642246
ATGGGGG2500.030.0795295
CAACGCA8900.029.5725735
TGCGATA404.778062E-429.37454274-75
CATGGGT3600.028.7217714
CTATAGT1005.3094256E-528.199564
ATAATAG856.803287E-427.6466273
CATGGGA8350.027.5804084
TATCAAC9900.027.0601842
GGTTATG909.418778E-426.1490671
ACGCAGA9750.026.0303617
GGGTACC1659.264659E-825.6359637
Produced by FastQC (version 0.11.2)