FastQCFastQC Report
Sun 14 Apr 2019
SRR6576568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249699
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4390.17581167725942035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.11093356401106934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA200.002163275470.4812243
CGCTAAA250.00523677156.384985
ATGGGAG2600.039.7586365
GTACATG14750.036.018831
TACATGG14700.035.5016752
CATGGGT2950.035.041514
ACATGGG14400.034.914313
GTATCAA7950.032.5266081
GCTATAC600.00414799931.3438191
ATGGGTA1658.54925E-1131.324995
TGCCCGC804.7609472E-429.3848321
TAGCGCG804.7777378E-429.3671784
GTGTATA856.787745E-427.6563111
GTATAAT1704.0290615E-927.6563111
TGGGTAC1704.0563464E-927.6396946
CGCGCGT856.811646E-427.6396947
GTAGCGC856.811646E-427.6396943
ACATACC856.811646E-427.6396943
CATGGGA7500.026.939494
GATATAG700.00880862226.8661331
Produced by FastQC (version 0.11.2)