FastQCFastQC Report
Sun 14 Apr 2019
SRR6576568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249699
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3480.13936779882979106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.1285547799550659No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3160.12655236905233902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7450.040.474511
GTGATCG608.771336E-539.191569
TACATGG13350.037.6716962
GATCGGG500.001700864837.593757
CATGGGG5550.037.255074
GTACATG13700.036.7977371
ACATGGG13550.036.414613
AACGCAG7900.036.2851076
ATGGGGG2750.035.8849455
ATCAACG8300.035.668773
CATGGGT3050.035.4367334
CAACGCA8500.034.2766535
TCAACGC8650.034.2255254
TATCAAC9950.030.2321872
ACGCAGA9200.030.1362937
AAACGAT953.7448954E-529.6792776
TAAACGA804.7749362E-429.370125
CGCAGAG9500.029.2080218
AACGATG856.8076566E-427.6424647
ATAAACG856.8076566E-427.6424644
Produced by FastQC (version 0.11.2)