FastQCFastQC Report
Sun 14 Apr 2019
SRR6576569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254308
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6000.23593437878478063No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5780.22728345156267202No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5580.21941897226984602No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4920.19346619060352013No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3310.13015713229627066No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT3080.12111298110952073No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3060.12032653318023814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.12032653318023814No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC2880.11324850181669473No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2770.10892303820564042No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG2730.10735014234707521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG352.9247077E-453.7456471
AGGGTAA901.3069439E-841.7775156
TCATATG500.001699588637.5997663
ACATGGG13050.036.73543
GTACATG13150.036.1199381
GTATCAA6300.035.8304371
TACATGG13250.035.826192
GGTAATC1107.656672E-834.1816068
TACTCTC550.002713133334.1816065
AAGGGTA1107.656672E-834.1816065
GTATTAT1001.3990466E-632.9192121
GTGCAAG1457.3305273E-1032.432721
CATGGGG7000.032.228374
GGTACCT1501.0604708E-931.3331388
TGGGTAC1501.0604708E-931.3331386
TGTAGTA600.004155074731.3331362
TCAACGC7300.030.2600844
AACGCAG7800.030.1280146
CAACGCA7450.029.650825
ATCAACG7550.029.2580933
Produced by FastQC (version 0.11.2)