FastQCFastQC Report
Sun 14 Apr 2019
SRR6576569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254308
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5840.22964279535051985No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5610.22059864416376992No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5300.20840870125988958No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5050.19857810214385704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.18874750302782453No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2820.11088915802884691No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2760.10852981424099911No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC2680.10538402252386869No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG2670.1049907985592274No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2660.10459757459458609No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT2620.10302467873602088No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2600.10223823080673829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCG250.0052335356.39416
GTATCAA6600.043.6149751
CGACGGT500.001700408837.596077
AGCGATC250.00170200937.5960786-87
CTTAGGT1750.034.903773
TCAACGC7950.034.8768584
CAACGCA8250.034.747885
TAGGTAT1502.7284841E-1134.463065
GGGCCGT550.002714441234.178246
AACGCAG8450.033.9254466
ATCAACG8250.033.6019973
TTAGGTA1554.1836756E-1133.351354
TACATGG12450.031.7075252
GTATATA600.00407369131.459981
GTACATG12650.031.335631
TATGGGA600.004157073331.3300574
AGTCTCG600.004157073331.3300577
TAGTATG600.004157073331.3300577
ACATGGG12900.029.8669683
GTCTTAG1751.6189006E-1029.6622661