FastQCFastQC Report
Sun 14 Apr 2019
SRR6576570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208907
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5220.24987195259134448No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3150.15078479897753547No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2480.11871311157596443No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2310.11057551925019267No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2220.10626738213654881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.10483133643200084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG200.002160496370.499992
ATATAGT450.001012049441.777786
CATGGGT3750.040.1066674
ATGGGTA2400.037.2083325
GTATCAA5950.037.126051
TGCCCGC651.4095347E-436.1538471
TACATGG13950.035.713262
GTACATG14300.035.4965021
GGGTACC2000.035.257
ACATGGG14050.035.1245543
GGTACCT2050.034.3902448
CATGGGG5100.032.25494
CTTAATG600.004153263331.3333343
CAACGCG300.004157978631.33333420-21
ATATACG1807.2759576E-1231.3333346
GCCCGTT600.004153263331.3333349
TACGCTA1859.094947E-1230.4864889
TTATTCT1252.3475513E-730.084
AACGCAG7300.029.616446
TGGGTAC2550.029.4901966
Produced by FastQC (version 0.11.2)