FastQCFastQC Report
Sun 14 Apr 2019
SRR6576570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208907
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4330.20726926335642176No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2590.12397861249264026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.1230212486896083No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2290.10961815544716069No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2270.10866079164412873No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2210.10578870023503283No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2190.10483133643200084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG450.001004056441.8451231
GTACATG12000.041.5835881
CATGGGG6100.040.0629234
TACATGG12600.039.5370642
ACATGGG12600.039.5370643
ATGGGGA3300.038.4525
GTCTAAC500.00169939437.597512
ACTAGGC500.00169939437.597513
TATACTG500.00169939437.597515
TAATACC757.4178006E-637.5975074
GTATCAA5650.035.8275681
CGCCGGT801.1546466E-535.247677
CTATACA550.00271282634.1795544
TAGTACT550.00271282634.1795544
CTTACAC550.00271282634.1795543
AGTATAG702.1797589E-433.5692065
TATAAGG702.1797589E-433.5692062
GTGTATA753.2357223E-431.383841
CCCCTTG902.585025E-531.331267
AACGCAG6900.030.6501456