FastQCFastQC Report
Sun 14 Apr 2019
SRR6576571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186466
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4290.23006875248034495No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2800.15016142353029505No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2460.13192753638733068No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2310.12388317441249343No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2300.12334688361417094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.12120172042088101No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2220.11905655722759109No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2030.10886703205946394No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2010.10779445046281896No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1890.10135896088294917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGC200.002162331770.4818956
TAGATTG503.004215E-546.9879345
ACATGGG11550.039.4617273
GTACATG12100.039.2529141
TACATGG11900.039.1222762
GTATCAA4750.034.6505661
CATGGGG4700.033.991274
CATGGGC2400.033.283124
CATGGGT2700.031.3252894
GAGTGCG600.004157350431.3252899
ATAGCCC600.004157350431.3252893
GTCTTAT804.7504302E-429.3911041
TAGTGCT804.7728824E-429.3674587
CCCTCGT650.006148841728.9156537
AGATAGT650.006148841728.9156536
TATGCAG650.006148841728.9156535
GATTGGC650.006148841728.9156537
AACGCAG5900.027.8741976
ATGGGTA1354.6100286E-727.8447025
TATTATG856.7727885E-427.6622142