FastQCFastQC Report
Sun 14 Apr 2019
SRR6576572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256300
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6320.24658603199375734No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5180.20210690596956693No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4830.1884510339445962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.18220834959032384No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4590.17908700741318767No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4300.16777214202106905No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4140.16152945766679672No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3500.13655872024970736No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3470.1353882169332813No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3280.1279750292625829No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3270.12758486149044088No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3050.11900117050331642No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2950.11509949278189621No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2940.1147093250097542No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2880.11236831837690207No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2880.11236831837690207No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2850.111197815060476No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2790.10885680842762388No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC2650.10339445961763559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA352.9335284E-453.71305594
GGACGAT405.669056E-446.9989287
AATAGAC405.669056E-446.9989287
GTATCAA9250.044.2216531
ATAGACG450.001012593941.7768258
TAACCGT250.001701338337.59914432-33
TTAACCG250.001701338337.59914432-33
GTACATG19400.034.6570631
TTAGAAG550.002713393234.1810383
TACATGG19850.033.8713842
ACATGGG20300.032.8760953
AACGCAG12850.031.8202846
AACCGTT300.004159316431.33261734-35
ACCGTTA300.004159316431.33261734-35
AGCTACG300.004159316431.33261768-69
CAACGCA13100.030.8542585
TCAACGC13100.030.8542584
ATCAACG13000.030.7300683
GTCTATG804.7603864E-429.3857961
CATGGGT4500.029.2437764
Produced by FastQC (version 0.11.2)