FastQCFastQC Report
Sun 14 Apr 2019
SRR6576572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256300
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6000.23410066328521262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.2200546234880999No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5300.20678891923527118No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4940.1927428794381584No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4190.16348029652750684No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4140.16152945766679672No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4040.15762777994537652No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3510.1369488880218494No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3390.13226687475614515No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3210.12524385485758877No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3110.12134217713616856No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3000.11705033164260631No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2960.11548966055403825No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2900.1131486539211861No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2790.10885680842762388No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2770.10807647288333982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.10495513070620367No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2630.10261412407335153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC250.005232443656.397193
GTATCAA9800.041.8366741
TAGTACT450.001012728341.7756964
TATACTC608.807484E-539.1647155
GTACATG18800.035.595451
TACATGG19400.034.400352
ACATGGG19350.034.2463533
GGTATCA5050.032.661841
AACGCAG12350.032.3465656
GCCTAAG753.217414E-431.4175821
CAACGCA13300.030.3894625
CATGGGG7900.030.3402634
GCCTAGT1252.296365E-730.1608771
ATCAACG13450.030.0505493
TCAACGC13550.029.8287724
CATGGGA10000.027.258644
CTAGTGG1057.401989E-526.8558063
AACACTC2104.9112714E-1126.8558067
CTATACT700.00882559526.8558044
CTCTATA700.00882559526.8558049
Produced by FastQC (version 0.11.2)