FastQCFastQC Report
Sun 14 Apr 2019
SRR6576573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240956
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6100.2531582529590464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.20252660236723719No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4750.1971314264845034No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4740.19671641295506231No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4660.19339630471953384No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4640.19256627766065174No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3900.1618552764820133No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3800.1577051411876027No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3600.14940487059878152No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3360.13944454589219607No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3290.13653945118610866No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3100.12865419412672852No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3030.1257490994206411No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3030.1257490994206411No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2940.12201397765567157No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG2700.11205365294908615No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2550.10582845000747024No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2520.10458340941914707No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2490.10333836883082388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA352.9343468E-453.708711
GTACATG19000.042.048271
TAGCACT450.001013910941.7647744
GTATCAA9600.041.6102641
TACATGG19450.040.8338052
ACATGGG19800.039.6290783
CATGGGT4750.039.5666284
ATGGGTA2800.035.2390255
ATGGGCG550.00271691234.171185
TAATAGG550.00271691234.171184
CATGGGA9450.033.8095784
AACGCAG11750.033.5895426
GAGTAAA753.2699105E-431.330081
CATGGGC3150.031.3235824
TGGGTAC2700.031.323586
TCTAGAC600.004160852631.3235783
ATCAACG12800.030.4670753
ATGGGAT2350.029.9906625
TCAACGC13050.029.8834174
CAACGCA13000.029.6369255
Produced by FastQC (version 0.11.2)