FastQCFastQC Report
Sun 14 Apr 2019
SRR6576574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711143
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17540.24664518950478315No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11480.16143026086173948No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10160.14286859323652204No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8790.12360383214065244No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8440.11868217784608721No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8240.11586980396347851No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT7700.10827639448043502No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7380.10377659626826109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.048.5645071
GTACATG34250.038.987081
TCAACGC19200.037.696344
ATCAACG19100.037.6476363
AACGCAG19650.037.5505876
CAACGCA19550.037.5022665
TACATGG35650.037.324142
ACATGGG35750.036.8096473
ACGCAGA23950.030.808737
CGCAGAG24600.029.8036298
CATGGGA15350.029.3929044
GTCTTAG2900.029.1835021
CATGGGG17100.029.1332824
TATCAAC25400.029.0622252
ATGGGAG8400.028.5345215
GTATAAT2104.9112714E-1126.8673531
GCAGAGT28200.026.1655719
GGGTACC6300.026.1100167
CATGGGT10450.025.635294
TGGGTAC6700.025.2526746
Produced by FastQC (version 0.11.2)