FastQCFastQC Report
Sun 14 Apr 2019
SRR6576575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643446
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16790.26093875787556375No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10280.15976476658491934No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9230.1434463808928799No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8830.13722985301019822No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8340.12961460635391314No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7520.11687072419441569No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT7240.11251915467653852No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7070.1098771303263988No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7000.1087892379469295No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6930.10770134556746022No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6750.10490390802025344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.10132940448771147No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT6470.10055233850237626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15250.044.385391
GTACATG37350.037.2519071
TACATGG37150.037.19942
ACATGGG38500.035.2708363
ATCAACG19800.033.460513
AACGCAG20000.033.360846
TCAACGC20300.032.636364
CAACGCA20600.032.1610765
CATGGGT10350.031.7787134
CATGGGG17400.028.6243234
GGTACCT5550.027.9382748
GGGTACC6000.027.4091437
ATGGGTA6700.027.35075
TGGGTAC5850.027.3087436
CGCAGAG24650.027.067628
ACGCAGA24700.027.0128277
TATCAAC25400.026.2785912
GTATTAT1807.4760464E-926.1140921
ACCGTTC909.5283636E-426.1039438
CATGGGA18200.024.0098924