FastQCFastQC Report
Sun 14 Apr 2019
SRR6576578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407609
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7150.17541320235814226No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4660.11432524797048152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12200.045.8589861
TACATGG23450.040.499142
GTACATG24050.039.879751
AACGCAG14550.038.7610666
ACATGGG24500.037.9818883
TCAACGC15200.037.103524
ATCAACG15150.036.9157563
CATGGGG8700.036.19374
CAACGCA15650.036.0366445
ACGCAGA16350.034.2063457
CGCAGAG16450.033.71278
TATCAAC18300.031.0864832
CATGGGA13500.030.6355974
GCAGAGT18600.030.068489
CATGGGT5200.029.8255214
ATATCGC650.00615098428.9217196
TATCGCT650.00615098428.9217197
ATGGGTA3100.028.80515
ATGGGAG6150.027.51095
GTCCTAC1057.3938485E-526.8657671
Produced by FastQC (version 0.11.2)