FastQCFastQC Report
Sun 14 Apr 2019
SRR6576578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407609
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5560.1364052314840938No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4680.11481591427078401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA205.672018E-447.00625682-83
GTATCAA10650.042.470851
TACATGG22450.034.543792
GTACATG23100.034.0622861
GTATAAT1404.947651E-1033.6543581
ACATGGG23350.033.614913
ATGGGAG5750.032.695995
AACGCAG13700.032.5915836
ATCAACG13950.032.3444253
CATGGGA11500.031.8785924
TCAACGC13750.031.4476014
CAACGCA13900.031.4463715
CATGGGT5300.027.490854
GTATATG1205.7202396E-627.484391
ACGCAGA15950.027.4013147
CGCAGAG16150.027.3529688
TATCAAC16650.027.0993842
ATGGGTA3700.026.6759535
GGGTACC3550.026.47597
ATCCTAC1101.0166852E-425.6366316
Produced by FastQC (version 0.11.2)