FastQCFastQC Report
Sun 14 Apr 2019
SRR6576579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374422
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6150.164253168884307No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4790.12793051690338708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.11030334755970535No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4030.10763256432581418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10750.041.577961
GTACATG22850.039.121491
ACATGGG22800.037.9184533
TACATGG23450.037.6688732
CATGGGT5300.036.3475954
AACGCAG12200.035.4367336
CTAGCAC801.1589449E-535.2394333
ATCAACG13500.032.7161183
TCAACGC13700.032.238514
CAACGCA13850.031.554325
ACGCAGA14450.029.5936957
CATGGGG8750.029.5340024
CGCAGAG14700.029.4336648
CATGGGA12000.028.5830974
GTAATAG700.00878402426.8849961
ATGGGTA3500.026.8526785
GTATACC700.00884146926.8490923
TATCAAC16400.026.644452
GCAGAGT16700.026.190299
CATGGGC4500.025.0591534
Produced by FastQC (version 0.11.2)