FastQCFastQC Report
Sun 14 Apr 2019
SRR6576580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521441
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.20366637836303625No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5830.11180555422377603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.053.509731
ATCAACG20000.040.888713
TCAACGC20450.039.9889534
CAACGCA20950.038.810235
TATCAAC22050.037.7375532
AACGCAG21800.037.5125776
GTACATG32400.033.162071
TACATGG32900.032.579772
ACGCAGA25400.032.01087
ACATGGG33050.031.853763
GGTATCA6750.031.41671
CCTATAC1052.063014E-631.3323423
CGCAGAG26200.031.0333718
GTAGTAC650.006071781329.000031
ACGTTGT1005.3191805E-528.1991069
GCAGAGT28850.028.019919
CATGGGA18850.027.426194
ATGGGGG4850.025.195085
ATGGGAG7550.024.8998765
ATGGGTA3300.024.2113555
Produced by FastQC (version 0.11.2)