FastQCFastQC Report
Sun 14 Apr 2019
SRR6576581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498322
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12210.24502229482142068No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7940.1593347273449697No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6750.13545458558923748No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6120.12281215760090866No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6120.12281215760090866No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5160.10354750542821708No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5140.1031461585079527No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5140.1031461585079527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG29950.036.10981
TACATGG30050.035.8331532
ACATGGG29850.034.9530223
GTATCAA13550.034.0079771
ATGGGAG3850.032.9593855
TAGACCG600.004159762531.331765
GGGTACC4850.031.0087537
ATTATAG804.778445E-429.3735283
CATGGGT7700.029.2972324
TGGGTAC5200.028.9216256
CATGGGG13600.028.3368154
ATCAACG15900.028.0803533
AACGCAG15800.027.960626
ATGGGTA5400.027.8504545
TCAACGC16050.027.8179194
CAACGCA16400.027.2242435
GGTACCT5550.026.2509358
CATGGGA13650.025.822884
ACCTAAG3350.023.861521
GTCCTAT2402.6011548E-1023.5106141
Produced by FastQC (version 0.11.2)