FastQCFastQC Report
Sun 14 Apr 2019
SRR6576581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498322
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12770.2562600085888241No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7420.14889970741809513No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6410.12863168794474256No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6230.12501956566236289No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5630.11297915805443066No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5460.1095677092321832No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5370.10776164809099338No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5330.10695895425046455No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5260.10555424002953913No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5160.10354750542821708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.10214279120729167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13300.036.4956631
GCATTAG651.3903614E-436.250361
GTACATG27200.035.5173531
TACATGG27450.034.934332
ACATGGG28150.033.3976753
GGGTACC4400.033.112087
GTCTAGT753.222122E-431.4169751
GGTACCT4750.030.6722438
ATACCGT1901.4551915E-1129.6857976
CATGGGG13650.029.268964
CTATAGT650.00607126329.0002881
CATGGGT7300.028.974154
ATCACGC650.00614624928.9275253
ATGGGCG1154.1883814E-628.6102245
TGGGTAC4700.028.0014936
TACCGTC1853.274181E-1027.9446337
AACGCAG16950.027.7300956
TAAGGGT1704.0618033E-927.6485374
TCAACGC17000.027.6485334
ACCGTCG1904.456524E-1027.209258
Produced by FastQC (version 0.11.2)