FastQCFastQC Report
Sun 14 Apr 2019
SRR6576586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238963
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4870.2037972405769931No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3380.14144449140661944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.11424362767457723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6100.049.3178441
GTACATG14950.037.1017841
TACATGG14950.036.787362
ATAGACT906.1769424E-736.5449754
ATGGGAG3750.035.0831765
TCAACGC8550.034.6215554
AACGCAG8650.034.221316
ATAACGC550.002716575534.171933
ATCAACG8700.034.024633
ACATGGG16050.033.959023
GTATAAG702.178191E-433.5757681
CAACGCA8900.033.2600335
GATCGTC851.7524642E-533.166877
TGATCGT902.5905201E-531.3242656
ATCGTCT902.5905201E-531.3242658
CATGGGA8800.030.968314
ACGCAGA9650.030.6750587
CGCAGAG9700.030.5169418
TATCAAC9800.030.2181872
GAGTAAA1102.9525145E-629.9129541
Produced by FastQC (version 0.11.2)