FastQCFastQC Report
Sun 14 Apr 2019
SRR6576586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238963
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5330.22304708260274606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.12470549834074732No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2800.11717295146110485No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2460.10294480735511355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG16950.042.1552
GTATCAA6750.041.837771
GTACATG17150.041.7157971
ACATGGG17550.040.169693
CATGGGG5100.037.7831344
GTATAAG757.3461742E-637.653991
CATGGGA9500.037.1041074
CATGGGT3700.036.836694
ATGGGTA2050.036.681785
ATGGGAC2100.035.8084075
ATGGGAG4150.032.842355
GTCCTAC902.5601566E-531.3783261
TCAACGC9550.030.0200084
ATCAACG9700.029.555783
AACGCAG9650.029.2280046
CAACGCA9650.029.2218865
CTTATCC650.00614548328.9221763
GTGTAAT1005.2544805E-528.2404941
GGTCCGT856.7433505E-427.6867581
TAATACT700.00882361126.8563084
Produced by FastQC (version 0.11.2)