FastQCFastQC Report
Sun 14 Apr 2019
SRR6576587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323205
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5510.17048003589053387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4520.1398493216379697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.12190405470212404No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3500.10829040392320663No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3320.10272118315001315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7400.042.6612051
GTACATG19400.040.0748861
TACATGG19400.039.267072
ACATGGG20600.037.2079353
CATGGGT5400.036.573864
ATGGGTA3300.034.1989335
TACACCG550.002707478134.1989335
CAACGCA9450.033.837045
GGTACCT2650.033.694128
GGGTACC2650.033.694127
AACGCAG9750.033.278196
TCAACGC9550.032.990334
ATCAACG9500.032.668983
CATGGGG6650.032.5275544
GTCGTAT300.004162060631.32961844-45
CTACCGA300.004162060631.32961816-17
GACCGTG650.006151274328.919657
TGGGTAC3250.027.4906796
ACGCAGA11800.027.4797957
CGCAGAG12000.027.0217958
Produced by FastQC (version 0.11.2)