FastQCFastQC Report
Sun 14 Apr 2019
SRR6576588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295501
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5950.20135295650437732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5000.16920416512972883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.12927198215911284No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3350.11336679063691832No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2980.1008456824173184No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2980.1008456824173184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7750.041.3089941
GTACATG18600.037.208461
TACATGG18900.036.3010642
ACATGGG19100.035.6749083
CTAGTAC702.1831445E-433.566053
AACGCAG10700.031.62116
ATACCTA753.2727743E-431.3283146
TAGTACT1052.0610842E-631.3283144
CATGGGA9650.031.165994
ATCAACG10500.030.8807663
CATGGGG6550.030.850024
CTATATA1102.9623152E-629.90432
CAACGCA11100.029.6348925
TCAACGC10950.029.6116964
ATATTAG804.7250284E-429.425061
ATGGGTG2250.029.239765
CATGGGT5050.028.8468634
TATCAAC11850.027.7592662
ATGGGAG4950.027.5309435
ATGGGTA3300.027.056275
Produced by FastQC (version 0.11.2)