FastQCFastQC Report
Sun 14 Apr 2019
SRR6576589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281801
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4360.15471911029414373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.12633028271723665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3260.11568447237589646No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3010.106812963758113No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2930.1039740810004223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC352.9135938E-453.7891121
GTATCAA7150.047.3946041
GTACATG19000.040.3772281
TACATGG18800.040.2488632
ACATGGG19100.039.3706133
ATGGGTA2550.038.7047845
CATGGGT3450.038.143854
AGACCGG500.001700084737.59893494
ATCAACG9500.035.125323
AACGCAG9700.034.4010856
CATGGGA10850.033.787064
TCAACGC9900.033.7061164
CAACGCA9950.033.536745
ATGGGAT3400.033.1755335
GGGTACC2350.031.9990967
TATCAAC10750.031.0409832
CATGGGG6800.029.028594
ACGCAGA12050.027.6921627
GGTACCT2750.027.344688
CGCAGAG12250.027.2400468
Produced by FastQC (version 0.11.2)