FastQCFastQC Report
Sun 14 Apr 2019
SRR6576590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253681
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5130.2022224762595543No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2990.11786456218636791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.1095864491231113No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2610.102885119500475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG15600.039.7811661
GTATCAA7350.039.0185051
TACATGG16200.037.7348062
ACATGGG16550.037.1989063
CCTACTA550.002709045534.1920781
CATGGGT3950.033.3133134
ATGGGTA2000.032.8968935
AACGCAG8700.032.4107326
ATCAACG8700.031.8705543
GTATAGT600.00414882631.3427351
CGTTGTT600.00416085931.32429
TCAACGC8950.030.9803164
CATGGGG6800.030.4088924
CAACGCA9400.029.9971685
TATGCTA650.006148086428.9203475
TACTACC650.006148086428.9203473
CATGGGA9250.027.9433084
GGCCAAT856.7894225E-427.6553541
ATGGGAG4050.026.6888375
TATCAAC10800.026.5595022
Produced by FastQC (version 0.11.2)