FastQCFastQC Report
Sun 14 Apr 2019
SRR6576590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253681
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5100.20103988867908906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3820.15058281857923927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.14309309723629282No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3370.13284400487226083No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2860.11274001600435192No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2680.10564449052156055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG250.005224705756.4181069
TATAGAC555.273694E-542.724143
GTATCAA9250.039.6842961
GTACATG17650.037.595871
TACATGG17950.036.9310722
CATGGGT3700.036.8351334
ACATGGG19000.034.8763853
GGTACCT2000.032.9105648
GGGTACC2050.032.088887
ATCAACG11200.031.8905163
TCAACGC11200.031.8905164
CAACGCA11750.030.7977395
AACGCAG11450.030.7837686
CATGGGC2600.030.7285164
TATCAAC12250.029.1685452
GTATTAG650.006106102428.9609261
CATGGGG6200.028.8043374
GTTATTC856.8042654E-427.6450273
ATGGGTA2750.027.3434475
CATGGGA9950.026.9226464
Produced by FastQC (version 0.11.2)