FastQCFastQC Report
Sun 14 Apr 2019
SRR6576591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360111
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5150.14301146035527934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.13384761920630028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA506.042683E-965.798645
CCCGCTA551.283297E-859.8169443
TACGGTC405.672076E-446.9990277
CCGCTAA708.5881766E-846.9990274
GTATCAA8800.044.365591
CGCAATA450.001012438241.7827152
ATCAACG10350.036.3277553
TCAACGC10850.034.6536644
CAACGCA11000.034.181115
ACCGTCC550.00271481834.181118
CAATACG550.00271481834.181114
AACGCAG11200.033.5707326
GTACATG18000.031.8814561
ACATGGG18000.031.593793
TACATGG18050.031.5106492
TATCAAC13150.029.311452
ACGCAGA13000.028.922487
ATACGAA650.006149254328.922486
AATACGA650.006149254328.922485
CGCAGAG13200.028.4842598
Produced by FastQC (version 0.11.2)