FastQCFastQC Report
Sun 14 Apr 2019
SRR6576591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360111
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.16966990733412754No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4600.12773839177364757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9600.045.197781
TCAACGC10900.039.237844
ATCAACG10900.039.2323883
CAACGCA10950.039.058675
AACGCAG11350.038.5103346
TATCAAC12900.033.887832
ACGCAGA13100.033.3658187
CGCAGAG13450.032.4975668
CTATAGA1102.882176E-630.0127561
GTACATG16400.029.9081821
TAGGCCC804.774935E-429.374484
TTACGGT650.00614916528.9225654
GCAGAGT15400.028.6878019
GGTATCA5150.028.389321
TACATGG17050.028.120712
ACATGGG17050.027.5616683
CGCAAGA1201.8331289E-423.5028482
ACGGACC1201.834856E-423.4995828
CGCGATA500.001759646923.4963214-15
CATGGGG8000.022.9120944
Produced by FastQC (version 0.11.2)