FastQCFastQC Report
Sun 14 Apr 2019
SRR6576592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426325
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13970.3276842784260834No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8310.19492171465431304No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6940.16278660646220608No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6860.16091010379405382No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6640.1557497214566352No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6490.15223127895384975No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6170.14472526828124083No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5820.13651556910807483No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5590.13112062393713717No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5580.13088606110361814No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5340.12525655309916145No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5280.12384917609804727No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5280.12384917609804727No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5160.12103442209581891No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5140.12056529642878086No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4720.11071365742098165No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4460.10461502374948689No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4420.10367677241541079No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4370.10250395824781564No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4370.10250395824781564No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4310.10109658124670146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC4900.042.200717
TGGGTAC4800.042.105746
GTATAGG901.26201485E-841.956521
TACATGG22850.040.522312
GTACATG23450.039.6529351
GGTACCT5200.038.862288
ACATGGG23900.038.1520543
GTATCAA9850.037.377511
ATGGGTA6350.032.5681465
AACGCAG11400.032.15916
CGGTGTC600.004159698731.3308329
CATGGGG13150.031.0962244
CATGGGT8350.030.9592444
GTACCTG6550.030.8524979
ATCAACG11900.030.8078733
TCAACGC11750.030.8011554
CAACGCA11950.030.2856525
TATACCG1205.8301866E-627.417694
GGGTGCG1205.835507E-627.4144789
ATGGGGG5350.027.2346655
Produced by FastQC (version 0.11.2)