FastQCFastQC Report
Sun 14 Apr 2019
SRR6576594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150681
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6130.4068197052050358No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2800.1858230301099674No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2730.18117745435721822No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2500.16591341974104235No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2460.16325880502518567No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2330.15463130719865145No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2310.15330399984072313No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2130.14135823361936808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.13604900418765473No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2040.13538535050869055No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1900.12609419900319216No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1810.12012131589251465No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1730.1148120864608013No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1730.1148120864608013No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1730.1148120864608013No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1690.11215747174494461No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1670.11083016438701629No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1640.10883920335012377No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1560.10352997391841043No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1560.10352997391841043No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1520.10087535920255374No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1510.10021170552358957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA255.544392E-575.198144
CCCGCTA255.544392E-575.198143
TGCACCG200.002159534770.498255
CGCTAAA301.3674819E-462.6651155
TATAGTG301.3674819E-462.6651155
ACATGGG10400.045.191193
GTACATG10700.044.8026281
GTATCAA5250.043.8655851
TACATGG11000.043.1534772
CATGGGG4950.039.87784
CATGGGT2150.039.3478624
ACGTGTT250.001700241737.5990784-85
AGTACTA250.001700241737.5990772-73
AACGCAG6400.035.9834866
ATGGGGG1601.8189894E-1235.2491265
GGGTACC1601.8189894E-1235.2491267
TCAACGC6700.034.3722844
GCATAAG550.002709883534.1809731
TTACATA550.002709883534.1809734
CAACGCA6800.033.866815
Produced by FastQC (version 0.11.2)