FastQCFastQC Report
Sun 14 Apr 2019
SRR6576599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312094
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12410.39763660948304036No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7490.23999179734310816No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6540.2095522502835684No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6360.20378475715649774No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6150.19705601517491525No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5420.17366562638179522No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5220.16725730068505001No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5090.16309188898216564No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC4970.1592468935641185No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4970.1592468935641185No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4590.1470710747403026No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4300.13777900248002206No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4300.13777900248002206No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4270.13681775362551024No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4240.13585650477099848No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4020.12880734650457876No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4020.12880734650457876No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3990.12784609765006696No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3970.12720526508039245No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3770.12079693938364723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3620.11599069511108832No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3600.1153498625414138No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.1153498625414138No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3480.11150486712336667No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3470.11118445083852942No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3460.11086403455369215No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3310.10605779028113324No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG3140.10061071343889982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAA250.0052356956.391256
GTACATG18050.044.3585661
TACATGG18750.042.3628852
ACATGGG18450.041.5165943
GTATCAA8550.039.6617771
CTTAGGT1101.7898856E-938.448583
AAGGGTA500.00170142437.5941665
TAGGTAT1152.7666829E-936.7769055
GTATATA651.3938779E-436.229511
AACGCAG9250.035.562056
TTAGGTA1204.1927706E-935.2445344
CATGGGG9000.034.461324
GGGTACC3550.034.4171947
CTTATCC550.00271605534.1765173
GGTACCT3650.033.4957288
TGGGTAC3700.033.0219046
GTCTTAG1755.456968E-1232.296021
TCAACGC10200.032.24994
GTATAGT2050.032.1131678
CAACGCA10250.032.092585
Produced by FastQC (version 0.11.2)