FastQCFastQC Report
Sun 14 Apr 2019
SRR6576600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423385
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9130.2156429727080553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.1247091890359838No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5230.12352823080647637No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4850.11455294826221997No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4760.1124272234491066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA156.899763E-493.997993
GTATCAA10500.048.3703881
GTACATG22850.041.7786981
AACGCAG12450.040.7702146
TCAACGC12550.040.4453474
TACATGG23900.040.1400032
CAACGCA12700.039.9676555
ATCAACG12950.039.1960753
ACATGGG24700.038.0558663
ATAGTAC801.15709245E-535.249253
ACGCAGA15300.033.1757627
TATCAAC15850.032.340172
CGCAGAG15950.031.823778
CATGGGA12050.031.2026544
CATGGGG9150.029.2780634
CATGGGC4550.027.8895154
GCAGAGT18550.027.6166619
CTATAGA1555.039692E-827.3058641
CATGGGT6850.026.0724334
GGGTACC3250.026.0302147
Produced by FastQC (version 0.11.2)