FastQCFastQC Report
Sun 14 Apr 2019
SRR6576601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381159
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8500.2230040481793687No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4790.12566934009166777No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4750.12461990927670605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.12330812075800388No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4320.1133385280158674No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4230.11097730868220348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12800.049.949981
CCGCTAA405.679611E-446.9870764
CCCGCTA450.001014473641.7662933
ATCAACG15750.039.677983
AACGCAG15850.039.4276476
TCAACGC16300.038.339154
CAACGCA16550.038.043925
TACATGG24350.036.489612
GTACATG24400.036.2221641
ACATGGG24000.036.2192043
ACGCAGA17400.035.915417
CGCAGAG17750.035.4719358
CATGGGT7500.034.457194
TATCAAC18300.034.42392
GCAAACG702.179071E-433.5798231
GCAGAGT19700.031.9607529
CGCTAAA600.004163123731.3247185
TACACCG600.004163123731.3247185
ACTAGAC753.2767467E-431.3247173
ATGGGTA4600.030.6437455
Produced by FastQC (version 0.11.2)