FastQCFastQC Report
Sun 14 Apr 2019
SRR6576601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381159
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7230.18968461980433363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.14455909476097903No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4740.1243575515729656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4420.11596210505327173No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4320.1133385280158674No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4310.11307617031212697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9650.047.299041
GTACATG21200.039.0647241
TACATGG21850.037.4276542
ACATGGG23350.035.4212273
AACGCAG13350.033.4410746
ATCAACG13200.033.1090623
TCAACGC13500.032.3733064
CATGGGT6800.031.7897264
ACGGACC902.5802608E-531.3495668
CAACGCA14050.031.1060225
ATGGGTA3800.030.916785
GGGTACC3100.030.318397
TATCAAC14950.029.5516342
ACGCAGA15400.028.98957
CGCAGAG15450.028.914658
ATGGGGG4100.028.6545775
GGTACCT3300.028.4996058
CATGGGG9200.028.0939454
CATGGGA10600.027.9301034
TATAGGG856.807948E-427.6468662
Produced by FastQC (version 0.11.2)