FastQCFastQC Report
Sun 14 Apr 2019
SRR6576602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403132
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.13667979718801782No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4940.12254050782374011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.042.105361
TACATGG19650.037.324452
ACATGGG19650.036.119193
ATGGGGG3600.035.252095
GTACATG20900.035.1139141
ATCAACG11000.034.611153
TCAACGC11000.034.611154
CATGGGG8500.033.7314154
CAACGCA11500.033.106315
AACGCAG12250.031.4591926
GCGATTG600.004159123631.3313069
TATCAAC12100.031.0839372
TACACCG804.7737805E-429.3767455
ATGGGGA5000.029.1417315
GTACTTG2350.028.0261
CGCAGAG14350.027.5104168
ACGCAGA14400.027.0885267
TACGCTA1701.2404598E-724.8807439
CATGGGA11250.024.2325524
TATACGC1751.6416925E-724.1698677
Produced by FastQC (version 0.11.2)