FastQCFastQC Report
Sun 14 Apr 2019
SRR6576602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403132
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.15875693321294265No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4450.1103856801246242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.039.5192571
AATACCG500.00170270237.591075
TTAGTAC550.002719743234.169463
GTACATG20550.030.9916081
ATCAACG12200.030.0383173
CAACGCA11900.030.0096765
TCAACGC12100.029.9019874
TACATGG20250.029.7126432
ACATGGG20000.029.3643823
AACGCAG13050.027.7252126
TATCAAC13350.027.4643732
CATGGGG8350.027.0115454
GGACCGT700.00883957326.8507636
ATGGGTA3050.026.1904985
GTATAAT2007.730705E-1025.9468611
CGCAGAG15250.023.725518
ACGCAGA15400.023.4944177
CATGGGT4900.023.014944
GTATAAG1652.5717563E-622.8732661
GTCATAC1252.3591377E-422.6445331
Produced by FastQC (version 0.11.2)