FastQCFastQC Report
Sun 14 Apr 2019
SRR6576603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844920
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14730.17433603181366283No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11090.1312550300620177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG250.005234609456.402592
GTATCAA15900.051.76871
ATCAACG20300.039.590613
CAACGCA20650.038.919585
TCAACGC20750.038.732024
AACGCAG21300.037.7318956
ACATGGG43150.037.5777173
GTACATG44100.037.5431251
TACATGG44350.037.0930252
CATGGGG21950.032.1180274
TATCAAC25600.031.7631762
ACGCAGA24950.031.6468757
CGCAGAG25750.030.6636718
CATGGGA17600.029.107574
CATGGGT10450.027.4350434
GCAGAGT30650.026.2215149
ATGGGAG9800.025.8976155
ATGGGGA12550.023.9678125
GGTACCT6500.023.8612238
GGTATCA6600.023.5177841
Produced by FastQC (version 0.11.2)