FastQCFastQC Report
Sun 14 Apr 2019
SRR6576606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441792
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10550.23880015935100682No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6110.13830037664783426No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5460.12358757062146894No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5020.11362813269592931No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4990.11294908011009706No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4930.11159097493843256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10750.045.5031931
GTACATG25250.038.5590171
TACATGG25250.038.5590172
ACATGGG25200.038.4184153
CATGGGT6200.034.8689544
TCAACGC13800.034.737124
ATCAACG13850.034.6117173
CAACGCA14150.033.87795
AACGCAG14150.033.87796
CATGGGG11800.032.2608454
ATGGGTA4350.031.3315245
TATACTG1452.6537236E-829.1707275
ACGCAGA16450.029.1411747
CGCAGAG16900.028.6433148
CATGGGA11450.028.3215074
TATCAAC17400.027.842292
ATGGGAG5800.027.550135
GCGTTAT700.00879864826.8768861
ATGGGGA7700.026.855595
GGGTACC4700.024.9985547
Produced by FastQC (version 0.11.2)