FastQCFastQC Report
Sun 14 Apr 2019
SRR6576606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441792
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9590.21707047660437492No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5670.12834093872229466No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5140.11634434303925831No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4930.11159097493843256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.10615855425177459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9850.043.564491
GTACATG23100.035.9275931
TACATGG23800.034.7603652
ACATGGG24000.033.687273
ATCAACG13050.032.0573843
AACGCAG13400.031.2165326
TCAACGC13450.031.1040064
CAACGCA13650.030.6447985
CATGGGA11550.029.3021244
CATGGGT6000.028.2032954
CATGGGG10150.027.7864974
GTACTAG700.00868959126.9456961
GTATAAG1458.3858504E-726.0165351
TATCAAC15900.026.0156172
ACGCAGA16050.025.7666477
ATCTATA1109.944403E-525.7208921
CGCAGAG16250.025.4495188
ATGGGTA3950.024.9874325
TAGTACT1151.3742437E-424.5246034
ATGGGAG5750.023.7044355
Produced by FastQC (version 0.11.2)