FastQCFastQC Report
Sun 14 Apr 2019
SRR6576607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405536
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9750.24042255188195374No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6120.15091138641205715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.14178765880217786No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4700.11589599936873668No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4680.1154028249033378No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4630.1141698887398406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4540.11195060364554564No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4540.11195060364554564No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4070.10036100370867199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA352.9273896E-453.7434169
GTACATG20850.037.707381
GTATCAA9300.036.4473081
TACATGG21450.036.3612752
ACATGGG22950.033.575263
CATGGGT5600.032.7216264
GTATTAA1605.820766E-1132.365971
GGTACCT3400.030.4282538
ATGGGTA3300.029.8994795
CCCCTAT953.704305E-529.7333321
TTCGGGT650.006129568428.94233194
GTCTTAG1154.134021E-628.6560361
ATGGGAG4800.028.3867055
CATGGGG8500.027.6381744
AACGCAG12400.026.9026415
CCCGTTA350.00884305226.85182426-27
TGGGTAC3400.026.2562646
CATGGGA12650.025.9995464
ATCAACG12700.025.8971862
CAACGCA12850.025.5948854
Produced by FastQC (version 0.11.2)