FastQCFastQC Report
Sun 14 Apr 2019
SRR6576608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196985
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4550.2309820544711526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.1456963728202655No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2580.1309744396781481No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2570.13046678681117851No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2560.12995913394420897No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2540.1289438282102698No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2420.12285199380663503No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2120.10762240779754804No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2070.1050841434627002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG459.994567E-441.8829771
GTACTAG459.994567E-441.8829771
GTACATG11850.037.3765831
ACATGGG11650.037.114883
CATGGGT2300.036.7816164
TACATGG12000.036.424022
ATGGGTA2150.034.97585
GGGGAGT851.7477656E-533.1755757
CATGGGG5650.031.6097664
GTATCAA5900.030.3474141
CATGGGA5500.029.0537594
TCAAAGC1802.3646862E-1028.7214453
ATGGGGA3550.027.8020655
TGGGTAC2550.027.6463136
AATTGCA856.7969825E-427.6463135
TGTTAGG856.7969825E-427.6463132
GTACAAG700.00871102826.9247721
TAGGGCT700.00881973726.8564194
GGTACCT2650.026.6030548
ATTGCAA909.491103E-426.1104056
Produced by FastQC (version 0.11.2)