FastQCFastQC Report
Sun 14 Apr 2019
SRR6576609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176988
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4490.253689515673379No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2610.14746762492372364No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2430.13729744389450133No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2330.13164734332271114No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2290.12938730309399507No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2040.11526205166451962No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1920.10848193097837142No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1890.10678690080683437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.10678690080683437No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1880.10622189074965534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG200.002162525470.4788065
CGATAAA250.00523496856.3830455
GATATAT558.769457E-751.2718051
GTATATC450.00101171841.7770271
CATGGGT2900.038.884864
TAATGCG250.00170294937.588718-19
ACATGGG12650.037.1429823
TACATGG13300.036.397842
GTACATG13800.035.0790751
GTACAAG702.177798E-433.570821
CATGGGG4900.033.5613374
ATGCGGT352.1858161E-433.56133720-21
ATGGGTA1900.032.1482285
GCGACCC300.004146000431.35048984-85
AACTATA753.2647824E-431.332772
TGCCCGC600.004151925431.3327661
CTCCTAA600.004151925431.3327661
TCTAAAC753.2702007E-431.3239153
TTACGCG300.004163248531.32391580-81
GGTTAGC600.00415767531.3239158