FastQCFastQC Report
Sun 14 Apr 2019
SRR6576610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135445
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3750.2768651482151427No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2270.1675957030528997No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1890.13954003470043191No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1650.12182066521466278No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1500.11074605928605707No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1480.10926944516224298No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1380.10188637454317251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA502.988876E-547.0169941
CATGGGT2650.035.471424
TACGCTA702.1749786E-433.5711639
GTTTTAG600.00414081231.3446621
CTATTAG600.00414081231.3446622
CCTATTA600.00414081231.3446621
GTATCAA5550.031.344661
GGTACCT1658.54925E-1131.3330888
ACTGTGC1052.04776E-631.3330888
GCGATAT300.004155579531.33308484-85
ATGGTGT600.00414830931.3330846
ATGGGTA2150.030.604415
TACATGG9300.030.3335442
ACATGGG9350.029.657523
ATACGCT804.7591163E-429.3747678
GTACATG9650.029.2333621
GGGTACC1802.3464963E-1028.7219967
ATACTCC856.7851716E-427.6468414
TATACGC856.7851716E-427.6468417
GTACTTG700.00879349126.8668521
Produced by FastQC (version 0.11.2)